nasopharyngeal bacterial colonization and gene polymorphisms of mannose-binding lectin and toll-like receptors 2 and 4 in infants鼻咽细菌殖民化和基因多态性mannose-binding凝集素和toll样受体2和4的婴儿.pdfVIP

Nasopharyngeal Bacterial Colonization and Gene Polymorphisms of Mannose-Binding Lectin and Toll-Like Receptors 2 and 4 in Infants 1 2,3 ¨ 1 1 Juho Vuononvirta , Laura Toivonen , Kirsi Grondahl-Yli-Hannuksela , Alex-Mikael Barkoff , Laura 4 2 2,3 1 Lindholm , Jussi Mertsola , Ville Peltola , Qiushui He * 1 Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland, 2 Department of Pediatrics, Turku University Hospital, Turku, Finland, 3 Turku Institute for Child and Youth Research, University of Turku, Turku, Finland, 4 Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland Abstract Background: Human nasopharynx is often colonized by potentially pathogenic bacteria. Gene polymorphisms in mannose- binding lectin (MBL), toll-like receptor (TLR) 2 and TLR4 have been reported. The present study aimed to investigate possible association between nasopharyngeal bacterial colonization and gene polymorphisms of MBL, TLR2 and TLR4 in healthy infants. Methodology/Principal Findings: From August 2008 to June 2010, 489 nasopharyngeal swabs and 412 blood samples were taken from 3-month-old healthy Finnish infants. Semi-quantitative culture was performed and pyrosequencing was used for detection of polymorphisms in MBL structural gene at codons 52, 54, and 57, TLR2 Arg753Gln and TLR4 Asp299Gly. Fifty- nine percent of subjects were culture positive for at least one of the four species: 11% for Streptococcus pneumoniae, 23% for Moraxella catarrhalis, 1% for Haemophilus influenzae and 25% for Staphylococcus aureus. Thirty-tw