mutational analysis of photosystem i of synechocystis sp. pcc 6803 the role of four conserved aromatic residues in the j-helix of psab突变分析集胞藻属的光系统i sp。6803年pcc的作用四个守恒的芳香j-helix psab的残留物.pdfVIP
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mutational analysis of photosystem i of synechocystis sp. pcc 6803 the role of four conserved aromatic residues in the j-helix of psab突变分析集胞藻属的光系统i sp。6803年pcc的作用四个守恒的芳香j-helix psab的残留物
Mutational Analysis of Photosystem I of Synechocystis sp. PCC 6803: The Role of Four Conserved Aromatic Residues in the j -helix of PsaB Wu Xu1*, Yingchun Wang2*, Eric Taylor 1, Amelie Laujac 1, Liyan Gao2, Sergei Savikhin3, Parag R. Chitnis4¤ 1 Department of Chemistry, University of Louisiana at Lafayette, Lafayette, Louisiana, United States of America, 2 Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, China, 3 Department of Physics, Purdue University, West Lafayette, Indiana, United States of America, 4 Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, United States of America Abstract Photosystem I is the light-driven plastocyanin-ferredoxin oxidoreductase in the photosynthetic electron transfer of cyanobacteria and plants. Two histidyl residues in the symmetric transmembrane helices A-j and B-j provide ligands for the P700 chlorophyll molecules of the reaction center of photosystem I. To determine the role of conserved aromatic residues adjacent to the histidyl molecule in the helix of B-j , we generated six site-directed mutants of the psaB gene in Synechocystis sp. PCC 6803. Three mutant strains with W645C, W643C/A644I and S641C/V642I substitutions could grow photoautotrophically and showed no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remained unaltered in these mutants. In contrast, the strains with H651C/L652M, F649C/G650I and F647C substitutions could not grow under photoautotrophic conditions because those mutants had low photosystem I activity, possibly due to low levels of proteins. A procedure to select spontaneous revertants from the mutants that are incapable to photoauto
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