multiple lineages of human breast cancer stemprogenitor cells identified by profiling with stem cell markers多个stemprogenitor血统的人类乳腺癌细胞和干细胞标记被分析.pdfVIP
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multiple lineages of human breast cancer stemprogenitor cells identified by profiling with stem cell markers多个stemprogenitor血统的人类乳腺癌细胞和干细胞标记被分析
Multiple Lineages of Human Breast Cancer Stem/ Progenitor Cells Identified by Profiling with Stem Cell Markers 1 2 1 1 1 Wendy W. Hwang-Verslues , Wen-Hung Kuo , Po-Hao Chang , Chi-Chun Pan , Hsing-Hui Wang , Sheng- 1 3 1 4 1 1,5,6 Ta Tsai , Yung-Ming Jeng , Jin-Yu Shew , John T. Kung , Chung-Hsuan Chen , Eva Y.-H. P. Lee *, King- Jen Chang2*, Wen-Hwa Lee1,6* 1 Genomics Research Center, Academia Sinica, Taipei, Taiwan, 2 Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan, 3 Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan, 4 Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, 5 Department of Development and Cell Biology, University of California Irvine, Irvine, California, United States of America, 6 Department of Biological Chemistry, University of California Irvine, Irvine, California, United States of America Abstract Heterogeneity of cancer stem/progenitor cells that give rise to different forms of cancer has been well demonstrated for leukemia. However, this fundamental concept has yet to be established for solid tumors including breast cancer. In this communication, we analyzed solid tumor cancer stem cell markers in human breast cancer cell lines and primary specimens using flow cytometry. The stem/progenitor cell properties of different marker expressing-cell populations were further assessed by in vitro soft agar colony formation assay and the ability to form tumors in NOD/SCID mice. We found that the expression of stem cell markers varied greatly among breast cancer cell lines. In MDA-M
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