multiple means to the same end the genetic basis of acquired stress resistance in yeast多个相同的目的的手段获得性耐压力在酵母的遗传基础.pdfVIP

Multiple Means to the Same End: The Genetic Basis of Acquired Stress Resistance in Yeast 1 1¤ 1 1 2,3 David B. Berry , Qiaoning Guan , James Hose , Suraiya Haroon , Marinella Gebbia , Lawrence E. Heisler2,3, Corey Nislow2,4, Guri Giaever2,3, Audrey P. Gasch1,5* 1 Laboratory of Genetics, University of Wisconsin–Madison, Madison, Wisconsin, United States of America, 2 Terrance Donnelly Centre for Cellular and Biomolecular Research, Toronto, Canada, 3 Department of Pharmaceutical Sciences, University of Toronto, Toronto, Canada, 4 Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada, 5 Genome Center of Wisconsin, University of Wisconsin–Madison, Madison, Wisconsin, United States of America Abstract In nature, stressful environments often occur in combination or close succession, and thus the ability to prepare for impending stress likely provides a significant fitness advantage. Organisms exposed to a mild dose of stress can become tolerant to what would otherwise be a lethal dose of subsequent stress; however, the mechanism of this acquired stress tolerance is poorly understood. To explore this, we exposed the yeast gene-deletion libraries, which interrogate all essential and non-essential genes, to successive stress treatments and identified genes necessary for acquiring subsequent stress resistance. Cells were exposed to one of three different mild stress pretreatments (salt, DTT, or heat shock) and then challenged with a severe dose of hydrogen peroxide (H O ). Surprisingly, there was little overlap in the genes required for 2 2