multilocus sequence subtyping and genetic structure of cryptosporidium muris and cryptosporidium andersoni茎子类型化序列和基因结构的隐孢子虫缪里斯和隐孢子虫andersoni.pdfVIP

Multilocus Sequence Subtyping and Genetic Structure of Cryptosporidium muris and Cryptosporidium andersoni 1 1 1 1 2 1 Rongjun Wang , Fuchun Jian , Longxian Zhang *, Changshen Ning , Aiqin Liu , Jinfeng Zhao , 3 1 1 1 4 5 Yaoyu Feng , Meng Qi , Helei Wang , Chaochao Lv , Guanghui Zhao , Lihua Xiao * 1 College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China, 2 Department of Parasitology, Harbin Medical University, Harbin, China, 3 School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, China, 4 College of Veterinary Medicine, Northwest A F University, Yangling, China, 5 Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America Abstract In this study, nine C. muris and 43 C. andersoni isolates from various animals in China were subtyped by a multilocus sequence typing (MLST) tool. DNA sequence analyses showed the presence of 1–2 subtypes of C. muris and 2–6 subtypes of C. andersoni at each of the four loci (MS1, MS2, MS3, and MS16), nine of which represented new subtypes. Altogether, two C. muris and 10 C. andersoni MLST subtypes were detected. Linkage disequilibrium analysis indicated although the overall population structure of the two parasites was clonal, the Chinese C. andersoni in cattle has an epidemic structure. Three and two clusters were produced in the C. muris and C. andersoni populations by Structure 2.3.3 analysis, with Chin