multilocus genotyping of human giardia isolates suggests limited zoonotic transmission and association between assemblage b and flatulence in children人类鞭毛虫隔离型茎表明有限的动物传染病的传播和关联组合b和儿童的肠胃气胀.pdfVIP

Multilocus Genotyping of Human Giardia Isolates Suggests Limited Zoonotic Transmission and Association between Assemblage B and Flatulence in Children 1,2 3 4 1 5 Marianne Lebbad , Ingvor Petersson , Lillemor Karlsson , Silvia Botero-Kleiven , Jan O. Andersson , 3 ¨ 2,6 Bo Svenungsson , Staffan G. Svard * 1 Department of Diagnostics and Vaccinology, Swedish Institute for Communicable Disease Control, Solna, Sweden, 2 Microbiology and Tumor Biology Centre, Karolinska Institutet, Stockholm, Sweden, 3 Department of Communicable Disease Control and Prevention, Stockholm County Council, Stockholm, Sweden, 4 Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden, 5 Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden, 6 Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden Abstract Background: Giardia intestinalis is one of the most common diarrhea-related parasites in humans, where infection ranges from asymptomatic to acute or chronic disease. G. intestinalis consists of eight genetically distinct genotypes or assemblages, designated A–H, and assemblages A and B can infect humans. Giardiasis has been classified as a possible zoonotic disease but the role of animals in human disease transmission still needs to be proven. We tried to link different assemblages and sub-assemblages of G. intestinalis isolates from Swedish human patients to clinical symptoms and zoonotic transmission. Methodology/Principal Findings: Multilocus sequence-based genotyping of 207 human Giardia isolates usi