multi-host model-based identification of armillifer agkistrodontis (pentastomida), a new zoonotic parasite from china多主机模型识别armillifer agkistrodontis(pentastomida),来自中国的一个新的人畜共患寄生虫.pdfVIP

Multi-host Model-Based Identification of Armillifer agkistrodontis (Pentastomida), a New Zoonotic Parasite from China 1 1 1 1 1 1 1,2 Shao-Hong Chen , Qin Liu , Yong-Nian Zhang , Jia-Xu Chen , Hao Li , Ying Chen , Peter Steinmann , Xiao-Nong Zhou1* 1 National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai, People’s Republic of China, 2 Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland Abstract Background: Pentastomiasis is a rare parasitic infection of humans. Pentastomids are dioecious obligate parasites requiring multiple hosts to complete their lifecycle. Despite their worm-like appearance, they are commonly placed into a separate sub-class of the subphylum Crustacea, phylum Arthropoda. However, their systematic position is not uncontested and historically, they have been considered as a separate phylum. Methodology/Principal Findings: An appraisal of Armillifer agkistrodontis was performed in terms of morphology and genetic identification after its lifecycle had been established in a multi-host model, i.e., mice and rats as intermediate hosts, and snakes (Agkistrodon acutus and Python molurus) as definitive hosts. Different stages of the parasite, including eggs, larvae and adults, were isolated and examined morphologically using light and electron microscopes. Phylogenetic and cluster analysis were also undertaken, focusing on the 18S rRNA and the Cox1 gene. The time for lifecycle completion was about 14 months, including 4 months for the devel