有哪些信誉好的足球投注网站molecular relatedness of methicillin-resistant s. aureus isolates from staff, environment and pets at university veterinary hospital in malaysia耐甲氧西林金黄色葡萄球菌分离株的分子亲缘从人员、环境和大学兽医宠物医院在马来西亚.pdfVIP
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molecular relatedness of methicillin-resistant s. aureus isolates from staff, environment and pets at university veterinary hospital in malaysia耐甲氧西林金黄色葡萄球菌分离株的分子亲缘从人员、环境和大学兽医宠物医院在马来西亚
Molecular Relatedness of Methicillin-Resistant S. aureus Isolates from Staff, Environment and Pets at University Veterinary Hospital in Malaysia 1,2 1 1 1 Erkihun Aklilu *, Zunita Zakaria , Latiffah Hassan , Chen Hui Cheng 1 Faculty of Veterinary Medicine Universiti Putra Malaysia, Serdang, Malaysia, 2 Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Kota Bharu, Malaysia Abstract Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a problem in veterinary medicine and is no longer considered as a mere nosocomial pathogen. We studied the occurrence of MRSA in veterinary personnel, cats and dogs and the environmental premises in University Veterinary Hospital (UVH). We found the prevalence of MRSA as follows: UVH 2/28 (7.1%) staff, 8/100 (8%) of the pets [5/50 (10%) of the dogs and 3/50 (6%) of the cats)], and 9/28 (4.5%) of the environmental samples. Antibiotic sensitivity tests (AST) show multi-resistance characteristics of the MRSA and the minimum inhibitory concentration (MIC) values for the isolates ranged from 1.5 mg to .256 mg/ml. Molecular typing by using multi-locus sequence typing (MLST), staphylococcal protein A typing (spa typing) and pulsed-field gel electrophoresis (PFGE) was conducted and the results from MLST indicated that an isolate from a veterinary personnel (PG21), typed as ST1241 belonged to the same clonal complex (CC) as the two isolates from two dogs (DG16 and DG20), both being typed as ST59. The PFGE results revealed that the two isolates from two veterinary personnel, PG21 and PG16 belonged to closely related MRSA strains with isolates from dog (DG36) and from environmental surface (EV100) respectively. The fact that
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