host transcription factors in the immediate pro-inflammatory response to the parasitic mite psoroptes ovis许多转录因子的直接促炎症反应寄生螨psoroptes羊属.pdfVIP

Host Transcription Factors in the Immediate Pro- Inflammatory Response to the Parasitic Mite Psoroptes ovis Stewart T. G. Burgess*, Tom N. McNeilly, Craig A. Watkins, Alasdair J. Nisbet, John F. Huntley Moredun Research Institute, Pentlands Science Park, Edinburgh, Scotland, United Kingdom Abstract Background: Sheep scab, caused by infestation with the ectoparasitic mite Psoroptes ovis, results in the rapid development of cutaneous inflammation and leads to the crusted skin lesions characteristic of the disease. We described previously the global host transcriptional response to infestation with P. ovis, elucidating elements of the inflammatory processes which lead to the development of a rapid and profound immune response. However, the mechanisms by which this response is instigated remain unclear. To identify novel methods of intervention a better understanding of the early events involved in triggering the immune response is essential. The objective of this study was to gain a clearer understanding of the mechanisms and signaling pathways involved in the instigation of the immediate pro-inflammatory response. Results: Through a combination of transcription factor binding site enrichment and pathway analysis we identified key roles for a number of transcription factors in the instigation of cutaneous inflammation. In particular, defined roles were elucidated for the transcription factors NF-kB and AP-1 in the orchestration of the early pro-inflammatory response, with these factors being implicated in the activation of a suite of inflammatory mediators. Conclusions: Interrogation of the host temporal response to P. ovis infestation has enabled the further identification of the mechanisms underlying the development of the immediate host pro-inflammatory response. This response involves key regulatory roles for the tr