hierarchical unilamellar vesicles of controlled compositional heterogeneity层次单膜囊泡的成分非均质性控制.pdfVIP

Hierarchical Unilamellar Vesicles of Controlled Compositional Heterogeneity 1 1 2 1 Maik Hadorn *, Eva Boenzli , Peter Eggenberger Hotz , Martin M. Hanczyc 1 Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark, 2 Department of Informatics, University of Zurich, Zurich, Switzerland Abstract Eukaryotic life contains hierarchical vesicular architectures (i.e. organelles) that are crucial for material production and trafficking, information storage and access, as well as energy production. In order to perform specific tasks, these compartments differ among each other in their membrane composition and their internal cargo and also differ from the cell membrane and the cytosol. Man-made structures that reproduce this nested architecture not only offer a deeper understanding of the functionalities and evolution of organelle-bearing eukaryotic life but also allow the engineering of novel biomimetic technologies. Here, we show the newly developed vesicle-in-water-in-oil emulsion transfer preparation technique to result in giant unilamellar vesicles internally compartmentalized by unilamellar vesicles of different membrane composition and internal cargo, i.e. hierarchical unilamellar vesicles of controlled compositional heterogeneity. The compartmentalized giant unilamellar vesicles were subsequently isolated by a separation step exploiting the heterogeneity of the membrane composition and the encapsulated cargo. Due to the controlled, efficient, and technically straightforward character of the new preparation technique, this study allows the hierarchical fabrication of compartmentali