hiv-1 epidemic in the caribbean is dominated by subtype b在加勒比海hiv - 1流行是由b亚型.pdfVIP

HIV-1 Epidemic in the Caribbean Is Dominated by Subtype B 1 1 1 1 2 Yuka Nadai , Lindsay M. Eyzaguirre , Anne Sill , Farley Cleghorn , Claudine Nolte , Manhattan 1 3 4 4 2 Charurat , Santiago Collado-Chastel , Noreen Jack , Courtenay Bartholomew , Jean W. Pape , Peter 5 1 1 Figueroa , William A. Blattner , Jean K. Carr * 1 Institute of Human Virology, School of Medicine, University of Maryland, Baltimore, Maryland, United States of America, 2 GHESKIO, Port-au-Prince, Haiti and Weill Medical College of Cornell University, New York, New York, United States of America, 3 Institute of Biomedical Studies, Santo Domingo, Dominican Republic, 4 Medical Research Foundation, Port of Spain, Trinidad and Tobago, 5 Ministry of Health, Kingston, Jamaica Abstract Background: The molecular epidemiology of HIV-1 in the Caribbean has been described using partial genome sequencing; subtype B is the most common subtype in multiple countries. To expand our knowledge of this, nearly full genome amplification, sequencing and analysis was conducted. Methodology/Principal Findings: Virion RNA from sera collected in Haiti, Dominican Republic, Jamaica and Trinidad and Tobago were reverse transcribed, PCR amplified, sequenced and phylogenetically analyzed. Nearly full genomes were completed for 15 strains; partial pol was done for 67 strains. All but one of the 67 strains analyzed in pol were subtype B; the exception was a unique recombinant of subtypes B and C collected in the Dominican Republic. Of the nearly full genomes of 14 strains that were subty