herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa单纯疱疹病毒1型穿透基底膜在人类鼻呼吸道粘膜.pdfVIP

Herpes Simplex Virus Type 1 Penetrates the Basement Membrane in Human Nasal Respiratory Mucosa 1 2 1,3 1 Sarah Glorieux *, Claus Bachert , Herman W. Favoreel , Annelies P. Vandekerckhove , Lennert 1 4 4 5 5 Steukers , Anamaria Rekecki , Wim Van den Broeck , Joline Goossens , Siska Croubels , Reginald F. Clayton6, Hans J. Nauwynck1 1 Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium, 2 Department of Otorhinolaryngology, University Hospital Ghent, Ghent, Belgium, 3 Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium, 4 Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium, 5 Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium, 6 Tibotec BVBA, Beerse, Belgium Abstract Background: Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial. Methodology/Principal Findings: A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated w