global assessment of genomic regions required for growth in mycobacterium tuberculosis全球评估所需的基因组区域结核分枝杆菌的增长.pdfVIP

Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis 1 2 3 4 4 Yanjia J. Zhang , Thomas R. Ioerger , Curtis Huttenhower , Jarukit E. Long , Christopher M. Sassetti , 5 1 James C. Sacchettini , Eric J. Rubin * 1 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, United States of America, 2 Department of Computer Science, Texas AM University, College Station, Texas, United States of America, 3 Department of Biostatistics, Harvard School of Public Health, Boston, Massachusetts, United States of America, 4 Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 5 Department of Biochemistry and Biophysics, Texas AM University, College Station, Texas, United States of America Abstract Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacteria. Genes, however, make up only a part of the complex genomes of important bacterial pathogens. Here, we use an unbiased analysis to comprehensively identify genomic regions, including genes, domains, and intergenic elements, required for the optimal growth of Mycobacterium tuberculosis, a major global health pathogen. We found that several proteins jointly contain both domains required for optimal growth and domains that are dispensable. In addition, many non-coding regions, including regulatory elements