有哪些信誉好的足球投注网站heat-induced structural changes affect ova-antigen processing and reduce allergic response in mouse model of food allergy燥热引起结构变化影响ova-antigen处理,减少过敏反应的食物过敏的小鼠模型.pdfVIP
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heat-induced structural changes affect ova-antigen processing and reduce allergic response in mouse model of food allergy燥热引起结构变化影响ova-antigen处理,减少过敏反应的食物过敏的小鼠模型
Heat-Induced Structural Changes Affect OVA-Antigen Processing and Reduce Allergic Response in Mouse Model of Food Allergy 1. 2 . 3 1 2 Jaroslav Golias , Martin Schwarzer * , Michael Wallner , Miloslav Kverka , Hana Kozakova , 2 1 1 1 3 Dagmar Srutkova , Klara Klimesova , Petr Sotkovsky , Lenka Palova-Jelinkova , Fatima Ferreira , Ludmila Tuckova1 1 Department of Immunology and Gnotobiology, Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic, 2 Department of Immunology and Gnotobiology, Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Novy Hradek, Czech Republic, 3 Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Salzburg, Austria Abstract Background and Aims: The egg protein ovalbumin (OVA) belongs to six most frequent food allergens. We investigated how thermal processing influences its ability to induce allergic symptoms and immune responses in mouse model of food allergy. Methodology/Principal Findings: Effect of increased temperature (70uC and 95uC) on OVA secondary structure was characterized by circular dichroism and by the kinetics of pepsin digestion with subsequent HPLC. BALB/c mice were sensitized intraperitoneally and challenged with repeated gavages of OVA or OVA heated to 70uC (h-OVA). Levels of allergen-specific serum antibodies were determin
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