genes of both parental origins are differentially involved in early embryogenesis of a tobacco interspecies hybrid父母的基因起源不同参与早期胚胎发生的烟草种间杂交.pdfVIP

genes of both parental origins are differentially involved in early embryogenesis of a tobacco interspecies hybrid父母的基因起源不同参与早期胚胎发生的烟草种间杂交.pdf

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genes of both parental origins are differentially involved in early embryogenesis of a tobacco interspecies hybrid父母的基因起源不同参与早期胚胎发生的烟草种间杂交

Genes of Both Parental Origins Are Differentially Involved in Early Embryogenesis of a Tobacco Interspecies Hybrid 1,2. 1. 1 1 1 1 1 Jun-E Zhang , An Luo , Hai-Ping Xin , Jing Zhao , Shi-Sheng Li , Liang-Huan Qu , Li-Gang Ma , 3 1 Stefan Scholten , Meng-Xiang Sun * 1 Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Science, Wuhan University, Wuhan, China, 2 College of Life Science, Jiangxi Normal University, Nanchang, China, 3 Biocenter Klein Flottbek, Developmental Biology and Biotechnology, University of Hamburg, Hamburg, Germany Abstract Background: In animals, early embryonic development is largely dependent on maternal transcripts synthesized during gametogenesis. However, in higher plants, the extent of maternal control over zygote development and early embryogenesis is not fully understood yet. Nothing is known about the activity of the parental genomes during seed formation of interspecies hybrids. Methodology/Principal Findings: Here, we report that an interspecies hybridization system between SR1 (Nicotiana tabacum) and Hamayan (N. rustica) has been successfully established. Based on the system we selected 58 genes that have polymorphic sites between SR1 and Hamayan, and analyzed the allele-specific expression of 28 genes in their hybrid zygotes (Hamayan x SR1). Finally the allele-specific expressions of 8 genes in hybrid zygotes were repeatedly confirmed. Among them, 4 genes were of paternal origin, 1 gene was of maternal origin and 3 genes were of biparental origin. These results revealed obvious

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