functional characterization of the dendritically localized mrna neuronatin in hippocampal neurons功能特性的枝晶的本地化mrna neuronatin海马神经元.pdfVIP

Functional Characterization of the Dendritically Localized mRNA Neuronatin in Hippocampal Neurons . . . Elaine L. Oyang , Bonnie C. Davidson , Winfong Lee , Michael M. Poon* Department of Biology, Harvey Mudd College, Claremont, California, Untied States of America Abstract Local translation of dendritic mRNAs plays an important role in neuronal development and synaptic plasticity. Although several hundred putative dendritic transcripts have been identified in the hippocampus, relatively few have been verified by in situ hybridization and thus remain uncharacterized. One such transcript encodes the protein neuronatin. Neuronatin has been shown to regulate calcium levels in non-neuronal cells such as pancreatic or embryonic stem cells, but its function in mature neurons remains unclear. Here we report that neuronatin is translated in hippocampal dendrites in response to blockade of action potentials and NMDA-receptor dependent synaptic transmission by TTX and APV. Our study also reveals that neuronatin can adjust dendritic calcium levels by regulating intracellular calcium storage. We propose that neuronatin may impact synaptic plasticity by modulating dendritic calcium levels during homeostatic plasticity, thereby potentially regulating neuronal excitability, receptor trafficking, and calcium dependent signaling. Citation: Oyang EL, Davidson BC, Lee W, Poon MM (2011) Functional Characterization of the Dendritically Localized mRNA Neuronatin in Hippocampal Neurons. PLoS ONE 6(9): e24879. doi:10.1371/journal.pone.0024879 ´ ´ Editor: Thierry Amedee, Centre national de la recherche scientifique, University of Bordeaux, France Received May 10, 2011; Accepted August 22, 2011; Published September 14, 2011