functional categories associated with clusters of genes that are co-expressed across the nci-60 cancer cell lines功能类别与集群相关的基因中的整个nci-60癌症细胞系.pdfVIP

Functional Categories Associated with Clusters of Genes That Are Co-Expressed across the NCI-60 Cancer Cell Lines 1 1 ´ 2 2 1¤ Barry R. Zeeberg *, William Reinhold , Rene Snajder , Gerhard G. Thallinger , John N. Weinstein , Kurt W. Kohn1, Yves Pommier1 1 Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health (NIH), Bethesda, Maryland, United States of America, 2 Institute for Genomics and Bioinformatics, Graz University of Technology, Graz, Austria Abstract Background: The NCI-60 is a panel of 60 diverse human cancer cell lines used by the U.S. National Cancer Institute to screen compounds for anticancer activity. In the current study, gene expression levels from five platforms were integrated to yield a single composite transcriptome profile. The comprehensive and reliable nature of that dataset allows us to study gene co- expression across cancer cell lines. Methodology/Principal Findings: Hierarchical clustering revealed numerous clusters of genes in which the genes co-vary across the NCI-60. To determine functional categorization associated with each cluster, we used the Gene Ontology (GO) Consortium database and the GoMiner tool. GO maps genes to hierarchically-organized biological process categories. GoMiner can leverage GO to perform ontological analyses of gene expression studies, generating a list of significant functional categories. Conclusions/Significance: GoMiner analysis revealed many clusters of coregulated genes that are associated with functional groupings of GO biological process categories. Notably, those categories arising f