a systematic proteomic study of irradiated dna repair deficient nbn-mice一个系统的辐照dna修复缺陷nbn-mice的蛋白质组学研究.pdfVIP

A Systematic Proteomic Study of Irradiated DNA Repair Deficient Nbn-Mice 1 ¨ 1 1 1 1 2 Anna Melchers , Lars Stockl , Janina Radszewski , Marco Anders , Harald Krenzlin , Candy Kalischke , 3 3 1 1 1 1. Regina Scholz , Andreas Jordan , Grit Nebrich , Joachim Klose , Karl Sperling , Martin Digweed , Ilja Demuth1.* ¨ ´ ¨ 1 Institut fur Humangenetik, Center for Biomedical Nanotechnology, Charite - Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany, 2 Medizinische Klinik ¨ ´ ¨ mit Schwerpunkt Hamatologie/Onkologie, Center for Biomedical Nanotechnology, Charite - Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany, ¨ ´ ¨ 3 Klinik fur Strahlenheilkunde, Center for Biomedical Nanotechnology, Charite - Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany Abstract Background: The NBN gene codes for the protein nibrin, which is involved in the detection and repair of DNA double strand breaks (DSBs). The NBN gene is essential in mammals. Methodology/Principal Findings: We have used a conditional null mutant mouse model in a proteomics approach to identify proteins with modified expression levels after 4 Gy ionizing irradiation in the absence of nibrin in vivo. Altogether, amongst ,8,000 resolved proteins,