a subtype-specific critical period for neurogenesis in the postnatal development of mouse olfactory glomeruli一个subtype-specific神经发生在产后发展的关键时期老鼠嗅小球.pdfVIP

A Subtype-Specific Critical Period for Neurogenesis in the Postnatal Development of Mouse Olfactory Glomeruli 1,2. 1. 1. 1 1 2 Yasuko Kato , Naoko Kaneko , Masato Sawada , Keishi Ito , Sousuke Arakawa , Shingo Murakami , Kazunobu Sawamoto1* 1 Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan, 2 Dpartment of Neuro- otolaryngology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan Abstract Sensory input is essential for the normal development of sensory centers in the brain, such as the somatosensory, visual, auditory, and olfactory systems. Visual deprivation during a specific developmental stage, called the critical period, results in severe and irreversible functional impairments in the primary visual cortex. Olfactory deprivation in the early postnatal period also causes significant developmental defects in the olfactory bulb, the primary center for olfaction. Olfactory bulb interneurons are continuously generated from neural stem cells in the ventricular-subventricular zone, suggesting that the olfactory system has plasticity even in adulthood. Here, we investigated the effect of transient neonatal olfactory deprivation on the addition of interneurons to the glomerular layer of the adult mouse olfactory bulb. We found that the addition of one subtype of interneurons was persistently inhibited even after reopening the naris. BrdU pulse-chase experiments revealed that the neonatal olfactory deprivation predominantly affected an early phase in the maturation of this neuronal subtype in the olfactory bulb. Subjecting the mice