a polyadenylation factor subunit implicated in regulating oxidative signaling in arabidopsis thaliana亚基聚腺苷酸化因素参与调节氧化信号在拟南芥.pdfVIP

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a polyadenylation factor subunit implicated in regulating oxidative signaling in arabidopsis thaliana亚基聚腺苷酸化因素参与调节氧化信号在拟南芥.pdf

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a polyadenylation factor subunit implicated in regulating oxidative signaling in arabidopsis thaliana亚基聚腺苷酸化因素参与调节氧化信号在拟南芥

A Polyadenylation Factor Subunit Implicated in Regulating Oxidative Signaling in Arabidopsis thaliana 1¤a 2.¤d 1,4.¤b 2 Jingxian Zhang , Balasubramanyam Addepalli , Kil-Young Yun , Arthur G. Hunt *, Ruqiang 3¤c 2 3 1,2,4 Xu , Suryadevara Rao , Qingshun Q. Li , Deane L. Falcone * 1 Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, Kentucky, United States of America, 2 Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America, 3 Department of Botany, Miami University, Oxford, Ohio, United States of America, 4 Department of Biological Sciences, University of Massachusetts, Lowell, Massachusetts, United States of America Abstract Background: Plants respond to many unfavorable environmental conditions via signaling mediated by altered levels of various reactive oxygen species (ROS). To gain additional insight into oxidative signaling responses, Arabidopsis mutants that exhibited tolerance to oxidative stress were isolated. We describe herein the isolation and characterization of one such mutant, oxt6. Methodology/Principal Findings: The oxt6 mutation is due to the disruption of a complex gene (At1g30460) that encodes the Arabidopsis ortholog of the 30-kD subunit of the cleavage and polyadenylation specificity factor (CPSF30) as well as a larger, related 65-kD protein. Expression of mRNAs encoding Arabidopsis CPSF30 alone was able to restore wild-type growth and stress susceptibility to the oxt6 mutant. Transcriptional profiling and single gene expression studies show elev