a snapshot of cnvs in the pig genome在猪的基因组中基因拷贝数异变的快照.pdfVIP

A Snapshot of CNVs in the Pig Genome ˜ Joao Fadista, Marianne Nygaard, Lars-Erik Holm, Bo Thomsen, Christian Bendixen* Group of Molecular Genetics and Systems Biology, Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Tjele, Denmark Abstract Recent studies of mammalian genomes have uncovered the extent of copy number variation (CNV) that contributes to phenotypic diversity, including health and disease status. Here we report a first account of CNVs in the pig genome covering part of the chromosomes 4, 7, 14, and 17 already sequenced and assembled. A custom tiling oligonucleotide array was used with a median probe spacing of 409 bp for screening 12 unrelated Duroc boars that are founders of a large family material. After a strict CNV calling pipeline, 37 copy number variable regions (CNVRs) across all four chromosomes were identified, with five CNVRs overlapping segmental duplications, three overlapping pig unigenes and one overlapping a RefSeq pig mRNA. This CNV snapshot analysis is the first of its kind in the porcine genome and constitutes the basis for a better understanding of porcine phenotypes and genotypes with the prospect of identifying important economic traits. Citation: Fadista J, Nygaard M, Holm L-E, Thomsen B, Bendixen C (2008) A Snapshot of CNVs in the Pig Genome. PLoS ONE 3(12): e3916. doi:10.1371/ journal.pone.0003916 Editor: Juergen Kroymann, Max Planck Institute for Chemical Ecology, Germany Received July 16, 2008; Accepted November 14, 2008; Published December 16, 2008 Copyright: 2008 Fadista et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.