a novel whole-cell biocatalyst with nad+ regeneration for production of chiral chemicals一种新颖的全细胞生物催化剂与nad +再生生产手性化学物质.pdfVIP

A Novel Whole-Cell Biocatalyst with NAD+ Regeneration for Production of Chiral Chemicals 1. 1. 1. 1 1 1 1 Zijun Xiao , Chuanjuan Lv , Chao Gao , Jiayang Qin , Cuiqing Ma *, Zhen Liu , Peihai Liu , Lixiang Li1, Ping Xu1,2* 1 State Key Laboratory of Microbial Technology, Shandong University, Jinan, People’s Republic of China, 2 MOE Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract Background: The high costs of pyridine nucleotide cofactors have limited the applications of NAD(P)-dependent oxidoreductases on an industrial scale. Although NAD(P)H regeneration systems have been widely studied, NAD(P)+ regeneration, which is required in reactions where the oxidized form of the cofactor is used, has been less well explored, particularly in whole-cell biocatalytic processes. Methodology/Principal Findings: Simultaneous overexpression of an NAD+ dependent enzyme and an NAD+ regenerating enzyme (H2O producing NADH oxidase from Lactobacillus brevis) in a whole-cell biocatalyst was studied for application in the NAD+-dependent oxidation system. The whole-cell biocatalyst with (2R,3R)-2,3-butanediol dehydrogenase as the catalyzing enzyme was used to produce (3R)-acetoin, (3S)-acetoin and (2S,3S)-2,3-butanediol. Conclusions/Significance: A recombinant strain, in which an NAD+ regeneration enzyme was coexpressed, displayed significantly higher biocatalytic efficiency in terms of the production