有哪些信誉好的足球投注网站a simple, inexpensive device for nucleic acid amplification without electricity—toward instrument-free molecular diagnostics in low-resource settings一个简单、廉价的核酸扩增设备没有electricity-toward instrument-free分子诊断在缺乏资源的环境中.pdfVIP
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a simple, inexpensive device for nucleic acid amplification without electricity—toward instrument-free molecular diagnostics in low-resource settings一个简单、廉价的核酸扩增设备没有electricity-toward instrument-free分子诊断在缺乏资源的环境中
A Simple, Inexpensive Device for Nucleic Acid Amplification without Electricity—Toward Instrument- Free Molecular Diagnostics in Low-Resource Settings Paul LaBarre*, Kenneth R. Hawkins, Jay Gerlach, Jared Wilmoth, Andrew Beddoe, Jered Singleton, David Boyle, Bernhard Weigl PATH, Seattle, Washington, United States of America Abstract Background: Molecular assays targeted to nucleic acid (NA) markers are becoming increasingly important to medical diagnostics. However, these are typically confined to wealthy, developed countries; or, to the national reference laboratories of developing-world countries. There are many infectious diseases that are endemic in low-resource settings (LRS) where the lack of simple, instrument-free, NA diagnostic tests is a critical barrier to timely treatment. One of the primary barriers to the practicality and availability of NA assays in LRS has been the complexity and power requirements of polymerase chain reaction (PCR) instrumentation (another is sample preparation). Methodology/Principal Findings: In this article, we investigate the hypothesis that an electricity-free heater based on exothermic chemical reactions and engineered phase change materials can successfully incubate isothermal NA amplification assays. We assess the heater’s equivalence to commercially available PCR instruments through the characterization of the temperature profiles produced, and a minimal method comparison. Versions of the prototype for several different isothermal techniques are presented. Conclusions/Significance: We demonstrate that an electricity-free heater based on exothermic chemical reactions and engineered phase change materials can successfully incubate isothermal NA amplification assays, and that the results of
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