a green fluorescent protein containing a qfg tri-peptide chromophore optical properties and x-ray crystal structure绿色荧光蛋白含有qfg tri-peptide发色团光学性质和x射线晶体结构.pdfVIP

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2017-09-05 发布于上海
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a green fluorescent protein containing a qfg tri-peptide chromophore optical properties and x-ray crystal structure绿色荧光蛋白含有qfg tri-peptide发色团光学性质和x射线晶体结构.pdf

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a green fluorescent protein containing a qfg tri-peptide chromophore optical properties and x-ray crystal structure绿色荧光蛋白含有qfg tri-peptide发色团光学性质和x射线晶体结构

A Green Fluorescent Protein Containing a QFG Tri- Peptide Chromophore: Optical Properties and X-Ray Crystal Structure 1. 2. 2 2 1 Jion M. Battad , Daouda A. K. Traore , Emma Byres , Jamie Rossjohn , Rodney J. Devenish , 3 2 1 Seth Olsen , Matthew C. J. Wilce *, Mark Prescott * 1 Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia, 2 The Structural Biology Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia, 3 School of Mathematics and Physics, The University of Queensland, Brisbane, Queensland, Australia Abstract Rtms5 is an deep blue weakly fluorescent GFP-like protein (lmax , 592 nm; lmax , 630nm; W , 0.004) that contains a 66Gln-Tyr- Abs Em F Gly chromophore tripeptide sequence. We investigated the optical properties and structure of two variants, Rtms5Y67F and Rtms5Y67F/H146S in which the tyrosine at position 67 was substituted by a phenylalanine. Compared to the parent proteins the optical spectra for these new variants were significantly blue-shifted. Rtms5Y67F spectra were characterised by two absorbing species (lmax , 440 nm and 513 nm) and green fluorescence emission (lmax , 440 nm; lmax , 508 nm; W , 0.11), Abs Ex Em F whilst Rtms5Y67F/