清斑胶囊对ICR鼠皮肤色素生成及抗氧化作用实验探究.docVIP

清斑胶囊对ICR鼠皮肤色素生成及抗氧化作用实验探究[摘要]目的：探讨清斑胶囊对黄体酮诱导的孕期全身色素加深小鼠血液和肝脏中的SOD、MDA、GSH的影响，以及对小鼠皮肤组织黑色素的影响。方法：制备黄体酮体内注射诱导小鼠全身色素加深模型，给药治疗后采用SOD、MDA、GSH试剂盒检测血清和肝脏中相关指标的变化；体外观察清斑胶囊对酪氨酸酶活性影响；免疫组化法观察对小鼠皮肤组织黑色素的影响。结果：清斑胶囊低中高三剂量组可以明显提高SOD、GSH活力，降低MDA含量；体外实验表明，清斑胶囊明显抑制酪氨酸酶活性，有效抑制皮肤组织黑色素的形成。结论：清斑胶囊具有良好的抗氧化作用及抑制小鼠皮肤黑素生成的作用。 [关键词]清斑胶囊；黄褐斑；抗氧化；黑色素 [中图分类号]Q591R332[文献标识码]A[文章编号]1008-6455（2011）10-1569-03 Experimental research of Qing-ban cupsule on the pigment formation of ICR mouse and the ablity of anti-oxidation HUANG Kai-fei1,2,WANG Mei-lan1,YANG Hui1,ZHANG Guo-dong1,XU Guang-hui1,HUANG Yi-qi1 （1.Xiamen Medicine Research Institute,Xiamen 361008,Fujian,China；2.Institute of Molecular Medicine,Huaqiao University,Quanzhou362021,Fujian,China） Abstract:ObjectiveTo investigate the effects of SOD,MDA,GSH in blood and liver on pigments-enhanced model induced by progesterone, as well as the impact of mouse skin melanoma after treated with Qing-ban cupsule.MethodsPrepare pigments-enhanced mice model induced by progesterone in vitro.SOD,MDA,GSH were detected by kit in serum and liver after drug treatment.The effects on tyrosinase activity were observed after drug treatment. Immuno-histochemistry was used to observe the effects of melanin in mouse skin tissue after drug treatment.Results Qing-ban capsule could significantly improve the SOD,GSH activity and decrease MDA content. In vitro experiment showed that the capsule significantly improved the activity of tyrosinase, inhibited the formation of melanin in skin tissue.ConclusionQing-ban capsule had good anti-oxidation activity and good treatment on skin melanogenesis. Key words:Qing-ban capsule; chloasma; anti-oxidation; melanin 黄褐斑是一种发生于面部的慢性难治性色素沉着性皮肤病。虽然针对该病的研究较多，但对其发病原因和病变机制的研究至今未取得实质性进展，且缺乏理想的疗法[1]。寻找更为有效的、低毒的祛斑药物成为广大科研工作者迫切的任务，中医美容学以传统医学理论为指导，采用多种多样的方法，在数千年间积累了丰富而宝贵的经验[2]。 清斑胶囊是本所自行研制的以大豆异黄酮、VC、VE、白芷、川芎、当归为主要原料的复方制剂。为探讨其抗黄褐斑作用机理，本研究以黄体酮攻击小鼠为实验动物模型，观察清斑胶囊抗氧化作用与治疗色素沉着之间的关系。 1材料 1.1动物：小白鼠（Institute of Cancer Researc