流体剪应力对内皮细胞蛋白C的活化以及蛋白C受体与血栓调节蛋白表达的影响_英文_.pdfVIP

26 2 Vol. 26 No.2 2009 4 Journal of Biomedical Engineering April 2009 Effects of Shear Stress on Protein Activation, EP R Expression And TM Expression in Endothelial ells 1,2 3 3 4 4 Jun Peng Huangqing hen Xiaoheng Liu Ruheng Li Xiaohong Zheng 1 ( Dep artment of Biolog ical Sciences , N ational University of Singap ore, Singap ore 117543) 2 (Academy of Integ rative Medicine, Fuj ian r aditional Chinese Medical College, Fuz hou 350108, China) 3 (Institute of Biomedical Eng ineering, West China Center of Medical Sciences , Sichuan University , Cheng du 61004 1, China) 4 ( College of Physics and Electronic I nf or mation, Dali University , Dali 671003, China) Abstract T he pr otein C anticoag ulant pathw ay plays a fundamenta l role in the contro l of coagulation sy stem and inflammato ry respo nse. t has been w ell established that physio lo gical levels of shear str ess induce endothelial struc- tur al chang e and modulate gene and pro tein ex pression. However , the r ole of shear str ess in pr otein C pathw ay r e- mains unknow n. n the present study , we evaluated the effect of shear str ess on the activ ation of pr otein C as w ell as on the ex pression of endo thelial protein C r eceptor ( EPCR) and thro mbo modulin ( T M) in human umbilical vein en- dothelial cells ( H U V ECs) which wer e exposed t o T N F-A alo ne, shear stress alo ne, and T N F-A under shear stress. We found: ( 1) Either T N F-A o r shear str ess alone sig nificantly r educed EPCR expr essio n and prot ein C act ivation in HU V ECs; and simultaneo us expo