持续静压力对人牙周膜成纤维细胞RANKLmRNA分泌活性影响.docVIP

持续静压力对人牙周膜成纤维细胞RANKLmRNA分泌活性影响[摘要]目的：研究持续静压力(continuously compressive pressure,CCP)对人牙周膜成纤维细胞(human periodontal ligament cells,HPDLs)核因子受体活化因子配体(receptor activator of nuclear factor kappa B ligand,RANKL)分泌活性的影响，初步探讨其在正畸性骨改建中的作用机制。方法：用组织块酶消化法培养获得人牙周膜成纤维细胞。建立空气静压力加载模型，分别对细胞加载0、50、100、150、200持续静压力0、0.5、1、1.5、2、4、6、12h，利用逆转录聚合酶链反应法(reverse transcription-polymerase chain reation,RT-PCR)检测RANKLmRNA分泌活性的变化。 结果：牙周膜细胞在持续静压力作用下RANKLmRNA表达增强，100Kpa加压1.5h时，RANKLmRNA的表达达峰值。结论：持续静压力可上调人牙周膜成纤维细胞RANKLmRNA的表达。 [关键词]牙周膜细胞；静压力；核因子受体活化因子配体；逆转录聚合酶链反应 [中图分类号]R783.5[文献标识码]A[文章编号]1008-6455（2009）02-0210-04 Effect of continuously compressive pressure on espression of RANKLmRNAin human periodontal ligament fibroblast in vitro WU Cheng-qiong,ZHOU Hong,WANG Xiao-rong （Department of Orthodontics,the Affiliated Stomatological Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710004,Shaanxi,China) Abstrcat:ObjectiveTo study the effect of continuously compressive pressure(CCP) on the expression of receptor activator of nuclear factor kappa B ligand(RANKL) in human periodontal ligament cells(HPDLs),and to investigate the role of continuously compressive pressure in alveolar bone rebuilding during orthordontic tooth movement. MethodsHPDLCs were isolated from human periodontal ligament by explanting enzymatic digestion with trypsin and collagenase. Estanblish a pressure model ,sustained static pressure 0Kpa、50 Kpa、100 Kpa、150 Kpa、200 Kpa were laid on HPDLCs for 0h、0.5h、1h、1.5h、2h、4h、6h、12h, respectively. The RANKL expression was identified by reverse transcription-polymerase chain reation(RT-PCR). Results The expression of RANKLmRNA significant increased after pressure exposed, especially in the group of 100 Kpa pressure lasted for 1.5 hours. ConclusionCCP can up-regulate the expression of RANKLmRNA in HPDLFs.100 Kpa pressure lasted for 1.5 hours was the suitable condition. Key words: human periodontal ligament fibroblasts;continuously compressive pressure;RANKL;RT-PCR