原花青素(OPC)抑制紫外线辐射后表皮黑素细胞黑素合成实验探究.docVIP

原花青素(OPC)抑制紫外线辐射后表皮黑素细胞黑素合成实验探究[摘要]目的：研究葡萄籽提取物原花青素（OPC）对正常表皮黑素细胞以及紫外线辐射后表皮黑素细胞黑素合成的影响。方法：培养的正常表皮黑素细胞和经10mJ/cm2紫外线辐射后的表皮黑素细胞分别以高中低（10、50、100μg/ml）三种浓度的OPC作用72 h，测定细胞增殖率、黑素含量，酪氨酸酶活性。结果：OPC对正常表皮黑素细胞的增殖和黑素合成无明显影响，但可呈浓度依赖性抑制紫外线辐射后的表皮黑素细胞的黑素合成，并能减轻紫外线辐射对细胞增殖的抑制作用。结论：天然寡聚体OPC对紫外线辐射的黑素细胞有光保护作用，它可明显抑制紫外线辐射后表皮黑素细胞的黑素合成，而对正常表皮黑素细胞的黑素合成无影响。 [关键词]黑素合成；原花青素；黑素细胞；表皮 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2008）06-0852-03 Lightening effect on ultraviolet-induced pigmentation of human melanocytes by proanthocyanidin-rich extract from grape seeds MA Hui-jun1, ZI Shao-xia 2,LIU Wen1, XIE Yan-fei3,WANG Yi-xia1, ZHAO Guang1 (1.Department of Dermatology, Airforce General Hospital of Chinese PLA, Beijing 100036, China.2.Department of Dermatology, Xuanwu Hospital of Capital Medical University, Beijing 100036,China. 3.Department of Dermatology, Hospital of Ophthalmology and Otorhinolaryngology, Botou 062150,Hebei, China) Abstract:ObjectiveTo study the effects of nature ologomers Proanthocyanidins on the pigment formation by normal human melanocytes and UVR-irradiated melanocytes.Methods Three different concentrations of Proanthocyanidins were incubated with normal human melanocytes and UV irradiated (10mJ/cm2 dose) human melanocytes for 72 h and then melanin content, reactivity of tyrasinase, rate of proliferation were measured.ResultsThere were no effects with melanogenesis and proliferation of normal melanocytes after expose of Proanthocyanidins.However, Proanthocyanidins can decrease the cellular melanin content and reactivity of tyrasinase of irradiated melanocytes with dose-dependent manner. Proanthocyanidins also can protecte proliferation of melanocytes after UV irradiation.ConclusionProanthocyanidins have sun-protection effects on melanocytes. It can inhibite melanogenesis and proliferation of epidermal melanocytes after UV irradiation. Key words:melanogenesis;Proanthocyanidins;melanocytes; epidermal 原花青素（OPC）是葡萄籽的提取物，具有很强的抗氧化和清除自由基能力，其抗氧化强度比维生素C高10～40倍[1]。在欧美等国