单因素方差研究优化桂枝茯苓胶囊中苦杏仁苷浸出率.docVIP

单因素方差研究优化桂枝茯苓胶囊中苦杏仁苷浸出率摘 要：目的：优化乙醇对桂枝茯苓胶囊苦杏仁苷的浸出率。方法：以苦杏仁苷浸率为考核指标，单因素方差分析最佳浸出苦杏仁苷乙醇浓度。用不同浓度乙醇处理桂枝茯苓胶囊，高效液相色谱法测定各乙醇处理液中苦杏仁苷的浓度。色谱柱:(4.6mm ID×150mm,粒径5μm);流动相:甲醇和0.2%磷酸水(24∶ 76)；检测波长:210nm;流速mL.min-1;柱温:室温;进样量15μL。结果：苦杏仁苷在0.3125～3.125μg范围内线性关系良好(r=0.9999),(n=6), 60％乙醇对苦杏仁苷浸出率最高。结论：该方法重现性好,可作为桂枝茯苓胶囊乙醇浸出苦杏仁苷的简便方法。 关键词：桂枝茯苓胶囊; HPLC法；单因素方差分析；苦杏仁苷 中图分类号：R283文献标识码：A文章编号：1673-7717(2011)12-2700-02 One–Way ANOVA Optimizing the Extract Technology of Amygdaloside in GZFL Capsules WANG hongyin1,HUANG Yanfen2 (1.Peoples Hospital of Lanxi,Lanxi 320000，Zhejiang,China； 2.Life Science Institute，Zhejiang Chinese Medical University,Hangzhou 310053, Zhejiang,China) Abstract:Objective:To optimize the extract technology of amygdaloside in GZFL capsules.Methods:Take amygdaloside content in extract as observation index, One Way ANOVA was used to study the factor of concentration of ethanol to extract amygdaloside in GZFL capsules. GZFL was treated with deferent concentration ethanol. Amygdaloside was determinated by HPLC. The column was Hypersil C18 (4.6 mm 150mm, 5(m). The mobile phase consisted of methanol and 0.2% phosphoric acid (24∶ 76) at a flow rate of 1mL.min-1. The detection wavelength was 210nm. Results: Amygdaloside had a good linearity in the range of 0.3125-3.125μg (r=0.9999). The average recovery was 99.9%, RSD=0.36%.60％ ethanol is the most suitable to extract Amygdaloside in GZFL capsules. Conclusion: The method is convenient, accurate, and has good reproducibility. It can be used to extract Amygdaloside in GZFL capsules. Key words:GZFL Capsules;Amygdaloside;HPLC ________________________________________ 收稿日期：2011-07-23 基金项目：浙江省教育厅基金资助项目 作者简介：王红英（1973－），女，浙江兰溪人，副主任中药师，学士，研究方向：医院药学。 通讯作者：黄燕芬(1974-),女，浙江金华人，副教授，研究方向：中药成分的分离与含量测定。桂枝茯苓胶囊组方源于东汉张仲景的《金匮要略》,由桂枝、茯苓、赤芍、牡丹皮、桃仁五味中草药组成,功效为活血化淤、缓消症块,其中苦杏仁苷为主要的有效成分之一。我们发现，桂枝茯苓胶囊对人宫颈癌Hela细胞增殖有抑制作用\[1\]，中药复方制剂体外细胞实验，给药量少，为了便于说明给药量，我们拟建立以苦杏仁苷作为桂枝茯苓胶囊对体外细胞实验的定量指标。分别用不同浓度乙醇处理桂枝茯苓胶囊，高效液相色谱法测定处理液中苦杏