体外培养兔鼻软骨细胞在静态牵张应力作用下增殖活性变化及其临床意义.docVIP

体外培养兔鼻软骨细胞在静态牵张应力作用下增殖活性变化及其临床意义[摘要]目的:探讨体外培养的不同年龄兔鼻软骨细胞在5kPa静态牵张应力作用下增殖活性的变化,从而为鼻软骨牵张术前正畸矫治唇腭裂伴发鼻畸形提供理论依据。材料与方法:将第4代体外培养的新生及6周龄新西兰白兔兔鼻软骨细胞置于细胞膜式静态牵张应力施加装置上培养,流式细胞仪检测5kPa静态牵张应力在0～12h内对兔鼻软骨细胞增殖活性的影响。结果:0～10h内5kPa静态牵张应力组软骨细胞增殖指数随着静态牵张应力作用时间的延长不断上升, 对体外培养兔鼻软骨细胞具有较大的促增殖作用,增殖指数峰值位于10h处;5kPa静态牵张应力对体外培养的新生兔兔鼻软骨细胞具有更大的促增殖作用。结论:5kPa静态牵张应力可促进体外培养的新生及六周龄新西兰白兔兔鼻软骨细胞增殖活性,提示我们鼻软骨牵张术前正畸方法不仅适用于临床矫治新生儿唇腭裂伴发鼻畸形,而且有可能用于矫治1岁左右婴儿甚至更大年龄患儿的唇腭裂伴发鼻畸形,从而为唇腭裂伴发鼻畸形的矫治开辟一条新的方法。 [关键词]静态牵张应力;兔鼻软骨细胞;增殖活性;流式细胞术 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455(2010)08-1152-04 Clinical Significance of tension force on proliferation of rabbit nasal chondrocytes in vitro BAI Ming-hai1, WU Han-jiang2,ZHANG Ting-ting3,JIANG Cui-chang1,LING Tian-you2 (1.Department of Orthodontics,Changsha Stomatology Hospital, Changsha 410005,Hunan,China;2.Department of Stomatology,Xiangya Second Hospital,Central South University,Changsha 410011,Hunan,China;3.Department of Laboratory,Xiangya Second Hospital,Central South University,Changsha 410011,Hunan,China) Abstract:ObjectiveThe purpose of this study was to investigate the effects of tension force on proliferation of rabbit nasalchondrocytes in vitro and its clinical significance.Materials and MethodsThe fourth-passage chondrocytes were harvested from the nose of neonatal and 6-week-old rabbits. A continuous static tension force was applied on the cells in vitro using a cellular static tension force device, and the proliferation of nasal chondrocytes were examined using a flow cytometry. The experimental group were divided into seven groups under continuous 5 kPa static distraction for 0 to 12 hours. ResultsThe results showed that the proliferation of nasal chondrocytes was promoted as the application time of 5kPa static tension force was prolonged (0～10 hours).The maximal proliferationappeared, when the nasal chondrocytes were cultured under 5kPa continuous static tension force for 10 hours (P 1.2 实验材料:Ⅱ型胶原蛋白酶(上海生工公司),胰蛋白酶(Sigma公司,美国),DME M培养基(Sigma公司