enzyme（酶）.docVIP

enzyme（酶） The rate of enzymatic reaction was affected by enzyme concentration and substrate concentration, and was also affected by temperature, pH, activators and inhibitors. (1) the effect of enzyme concentration on the rate of enzymatic reaction M from the relation diagram of formula and the enzyme concentration and the velocity of enzymatic reaction can be seen: the velocity of enzymatic reaction and enzyme molecules is proportional to the concentration of. When the concentration of substrate molecules is enough, the more enzyme molecules, the faster the substrate conversion. But in fact, when the enzyme concentration is high, this relationship is not maintained, and the curve gradually tends to be smooth. According to the analysis, this may be caused by high concentrations of substrate entrainment clamps, which are caused by many inhibitors. (2) the effect of substrate concentration on the rate of enzymatic reaction In biochemical reaction, if the concentration of enzyme is fixed and the initial concentration of substrate is lower, the rate of enzymatic reaction is directly proportional to the concentration of substrate, that is, with the increase of substrate concentration. When all enzymes were combined with substrates to produce intermediates, the concentration of intermediate products did not increase even when substrate concentration was increased, and the rate of enzymatic reaction was not increased. It can also be concluded that the enzyme reaction rate is proportional to the initial concentration of the enzyme under the same concentration of substrate. The initial concentration of enzyme is large, and its enzymatic reaction rate is large. In actual determination, the enzyme reaction rate was not increased or even inhibited even with the increase of substrate concentration, even if the enzyme concentration was high enough. The reason is that the high concentration substrate reduces the effective concentration of water and reduces the molecular diffusion,