elisa测定错误结果的原因分析（Analysis of the cause of error result of ELISA determination）.docVIP

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elisa测定错误结果的原因分析（Analysis of the cause of error result of ELISA determination）.doc

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elisa测定错误结果的原因分析（Analysis of the cause of error result of ELISA determination）

elisa测定错误结果的原因分析（Analysis of the cause of error result of ELISA determination） ELISA, enzyme-linked immunosorbent assay (ELISA), is a commonly used solid-phase enzyme immunoassay. Engvall and Perlmann were first used in the quantitative determination of IgG in 1971 and named enzyme linked, immunosorbent, assay (ELISA). The basic principle of ELISA is that: Make antigen (or antibody) bind to certain solid carrier surface, and maintain its immunity activity; To make antigen (or antibody) linked to an enzyme labeled antigen (or antibody), and the enzyme labeled antigen (or antibody) not only retains its immune activity, but also retains its enzyme activity; The determination of the tested samples (antibody or antigen) and enzyme labeled antigen (or antibody) according to the different reaction steps and solid carrier surface antigen or antibody, and then the washing method of separating antigen antibody complexes formed a solid carrier and other substances, and finally the amount of enzyme in the solid phase carrier on the specimen the amount of antibody or antigen detection in a certain proportion; adding the substrate, the substrate catalyzed by the enzyme into the non-ferrous products, qualitative or quantitative analysis based on its color reaction depth, in order to understand the measured antibody or antigen content in samples. The ELISA method is widely used in various antigens and antibody assays. But there are many influencing factors in ELISA determination, and there are some technical requirements in the operation. In the clinical examination, except for the normal reaction, sometimes some erroneous results can be seen (i.e. false positive or false negative results). The main reasons for the error in ELISA determination are: Specimen factor; Reagent factor; Operating factors. In this paper, the effect of specimen factors on ELISA determination is discussed as follows. The serum is the most commonly used ELISA specimens, the plasma can be regarded as equal an