大鼠少突胶质细胞前体细胞的生后发育特点及围生期电生理特性的研究.docVIP

文章编号：2008217 大鼠少突胶质细胞前体细胞的生后发育特点及围生期电生理特性的研究 陈鹏慧，蔡文琴，*王丽雁（第三军医大学基础医学部神经生物学教研室，重庆市神经科学研究所，重庆 400038） 摘 要：目的 研究少突胶质细胞前体细胞（OPC）的发育特点及围生期电生理特性。方法 应用免疫组化及Western blot，观察生后不同发育阶段大鼠大脑皮质、海马等区域OPC细胞数量。应用脑片膜片钳记录P7d大鼠皮层白质和海马OPC电生理学特性。结果 P0d、P7d、P14d、P21d、P35d、P50d、18个月大鼠大脑皮层及海马区域均有OPC细胞表达。在发育生后7d，细胞数量密度最高，成年大鼠也有较高数量OPC的分布。P7d大鼠海马OPC有小的内向Na+电流，而白质区域OPC仅表现外向K+电流和细胞膜被动反应。结论 P7d大鼠皮层及海马区域OPC细胞数量及NG2蛋白表达量最大，海马和白质区域的OPC表现电生理学特性的异质性。Study on the postnatal development and perinatal electrophysiological character of oligodendrocyte precursor cell in rats CHEN Peng-hui, CAI Wen-qin, WANG Li-yan (Department of Neurobiology, Third Military Medical University, Chongqing Institute of Neuroscience, Chongqing 400038) Abstract：Objective To observe the postnatal development and perinatal electrophysiological character of oligodendrocyte precursor cell (OPC) in rats. Methods Immunohistochemistry and western blot were applied to determine the expression of NG2 OPC in cerebral cortex and hippocampus of various developmental stages in rats. Electrophysiological characteristics of OPC were also recorded in slices of 7d rats. Results The majority of hippocampal and cerebral OPC showed a stellate morphology, a small cell body with a little process. Total populations of the NG2 immunopositive OPC were numerous at P7d in cerebral cortex and hippocampus. OPC expressed in adult rats with slightly more quantity. Moreover, OPC in hippocampus of P7d rats typically exhibited small inward sodium current and weak active responses. Therefore only outward potassium current and inactive responses were recorded in white matter OPC of P7d rats. Conclusions Total populations of OPC and relative optical density of NG2 are the highest level in P7d rats during the postnatal developmental stages. OPC in cerebrum and hippocampus of P7d rats displayed electrophysiological heterogeneity. Key words：oligodendrocyte precursor cell (OPC); NG2; development; patch clamp 神经胶质细胞约占中枢神经系统细胞总数的90%，其主要种类有星状胶质细胞、少突胶质细胞和小胶质细胞等。近年来研究发