草甘膦中甲醛分析hplc法.docxVIP

ANALYSIS OF FORMALDEHYDE IN GLYPHOSATE WETCAKE, GLYPHOSATE ISOPROPYLAMINE SALT AND ROUNDUP? SAMPLES草甘膦滤饼、草甘膦异丙胺盐和Roundup样品中甲醇的分析1. Principle of Method 原理This method describes a liquid chromatographic procedure for the selective determination of formaldehyde in glyphosate wetcake, glyphosate isopropylamine salt, and Roundup? samples 本方法使用液谱法测定草甘膦滤饼、草甘膦异丙胺盐及roundup样品中甲醛的含量The Hantzch reagent is used to react with formaldehyde present in aqueous glyphosate solutions. The resulting derivative, diacetyldihydrolutidine or DDL, is determined by reversed phase HPLC with UV detection.使用Hantzch试剂与甲醛反应，生成“diacetyldihydrolutidine”或者DDL，能够被反向色谱柱分离，用紫外检测器检测。Quantitation is based on the area of the DDL peak. This response is compared to the responseof external standards prepared in the same manner as the samples.使用相同操作条件下的样品及表样中DDL峰面积进行定量计算。2. Safety安全Several of the solvents and reagents for this method are hazardous chemicals and should beused only with proper ventilation.该方法的数种溶剂和试剂是危险化学品，需要在通风处处理。3. Range and Sensitivity量程与精度Range量程This method has been validated for the range of 0.3 - 14 ug/mL formaldehyde in dilutedsamples of glyphosate wetcake, glyphosate isopropylamine salt, and Roundup?该方法被证实可用于甲醛浓度在0.3-14 ug/mL的草甘膦稀释溶液。SensitivityAnalytical response was found to be linear over the range of 0.3 - 14. The detectionlimit of the method is 1 ppm under these conditions.本方法在0.3-14 ug/mL浓度下线性相关，本方法的检出限为1ppm。4. Interferences干扰Formaldehyde generating substances such as N-isopropylhexahydrotriazine will interfere withthis method.甲醛的衍生物如N-isopropylhexahydrotriazine会产生干扰。Any compound with the same retention time as DDL, that responds at 412 nm will interferewith this method.任何与DDL有相同的保留时间，并且在412nm出可被紫外检测到的物质能够产生干扰。The presence offormaldehyde in reagents will cause a high background level, visible inreagent blanks.有甲醛的试剂，甲醇容易产生高亮背景噪声。Formaldehyde contamination from Bakelite caps must be avoided.瓶塞应避免被甲醛污染。5. Precision and Accuracy精密度和准确度Precision精密度The pooled coefficient of variation of