pcr-sscp技术_分子生物学实验-舒鸣读研记录（The experimental technique of molecular biology _ PCR-SSCP Shu Ming graduate school record）.docVIP

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pcr-sscp技术_分子生物学实验-舒鸣读研记录（The experimental technique of molecular biology _ PCR-SSCP Shu Ming graduate school record）.doc

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pcr-sscp技术_分子生物学实验-舒鸣读研记录（The experimental technique of molecular biology _ PCR-SSCP Shu Ming graduate school record）

pcr-sscp技术_分子生物学实验-舒鸣读研记录（The experimental technique of molecular biology _ PCR-SSCP Shu Ming graduate school record） The experimental technology of molecular biology _ - PCR-SSCP Shu Ming graduate record Baidu space | Baidu home page | login Molecular biology experiments - Shu Ming graduate records, DNA extraction, purification, recovery, PCR experiments, gel electrophoresis, polypropylene electrophoresis, enzyme digestion experiments (single, double or multiple) sensory cells, transformation, screening, plasmid DNA extraction, recovery, sequencing Principle and procedure of common molecular biology experiment content, analysis of the common problems and some experimental experience home page blog album | personal files | friends see article PCR-SSCP technology, at 21:37 on Tuesday November 27, 2007, sharply differentiated the molecules with different conformations. The authors called the method a single strand conformation polymorphism (Single-Strand, Conformation, Polymorphism, SSCP) analysis. In subsequent studies, the authors SSCP is used to check the amplified products of PCR gene mutation, so as to establish the PCR-SSCP technology, to further improve the mutation detection method is simple and sensitive. The basic process is: DNA amplification of target PCR; the specific PCR amplification The product is denatured and then rapidly refolded to make it a single stranded DNA molecule with a certain spatial structure DNA chain of non denaturing polyacrylamide gel electrophoresis; finally by autoradiography, silver staining or ethidium bromide colorimetric analysis results. If it is found that the single stranded DNA with mobility compared with the normal control change, you can determine the chain. A change in the image results in a base mutation in the DN- segment. This method is simple, fast, sensitive, and does not require special use Special instruments are suitable for clinical trials, but they also have disadvantages. For example, they can only be used as a