以Fe(Ⅲ)为电子受体聚磷菌筛选、鉴定及聚磷特性.docVIP

以Fe(Ⅲ)为电子受体聚磷菌筛选、鉴定及聚磷特性作者简介：任丽平（1971），女，副教授，博士生，主要从事资源微生物和水污染控制研究，(Email)xhrlph@163.com。 张智（通信作者），男，教授，博士生导师，(Email)zhangzhicq@126.com。摘要：结合平板分离法、poly-P和PHB染色以及柠檬酸铁还原实验，从污水处理厂活性污泥中分离获得一株以Fe(Ⅲ)为电子受体厌氧聚磷的聚磷菌株AP3, 其16S rDNA与Pseudomonas mosselii ATCCBAA-99的同源性为99%。菌株AP3于柠檬酸铁液体培养基和无铁液体培养基中厌氧避光、27 ℃静置培养，在含有Fe(Ⅲ)的培养基中比在无Fe(Ⅲ)的培养基中生长好；菌株AP3在无Fe(Ⅲ)的培养基中，表现出典型的厌氧释磷特征，释磷量为3.68 mg·L-1；在含有Fe(Ⅲ)的培养基中，先厌氧释磷，释磷量为6.62 mg·L-1， 10 h之后开始厌氧聚磷，聚磷量为5.89 mg·L-1。菌株AP3能以Fe(Ⅲ)为电子受体，具有厌氧聚磷特征。 关键词：聚磷菌；筛选；鉴定；氧化铁；电子受体 中图分类号：X172文献标志码：A文章编号：16744764（2012）03014605 Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on PhosphorusAccumulating Characteristics REN Liping1，2，ZHANG Zhi1，TANG Yun2 （1. College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, P. R. China； 2. College of Biological Sciences, China West Normal University, Nanchong 637002, P. R. China） Abstract:A phosphorus accumulation organism strain AP3 with Fe (Ⅲ) as electronic acceptor was screened from activated sludge through such processes as dilutionplate method, polyP, PHB staining and ferric citrate reduction. Its 16S rDNA is identical to that of Pseudomonas mosseliisp ATCCBAA99 at 99% level. By comparing the AP3 being cultured in ferric citrate fluid medium and the one in nonferric fluid medium at 27 ℃, it was found that ferric citrate fluid medium was beneficial for the AP3. AP3 cultured in nonferric fluid medium showed significant anaerobic and phosphorus releasing characteristic, the phosphorus releasing amount of which was 3.68 mg·L-1. AP3 cultured in ferric fluid medium was also characterized by anaerobic and phosphorus releasing property at the first stage, the phosphorus releasing amount of which was 662 mg·L-1，which was higher than the former; And at the second stage which started from the 10h, it was characterized by anaerobic and phosphorus accumulating property, the phosphorus accumu