膀胱出口部分梗阻大鼠逼尿肌中糖原变化及其意义李海洋1钟甘平1.docVIP

膀胱出口部分梗阻大鼠逼尿肌中糖原变化及其意义 李海洋 ，钟甘平1 ，王志平1，王道英2 ，史庭恺1 ，马俊林1 （1.兰州大学第二医院泌尿外科,甘肃 兰州730030 2.甘肃武威肿瘤医院，甘肃 武威 733000） 摘要 目的探讨大鼠膀胱出口部分梗阻(Partial Bladder Outlet Obstruction,PBOO)后逼尿肌中糖原变化及其意义。方法 建立大鼠PBOO模型，每周测量一批大鼠尿动力学数据后获取膀胱组织,采用PAS染色观察逼尿肌细胞中糖原变化，根据含量和分布的不同分为0分、1分、2分、3分共4个等级（组），然后分析这种变化和膀胱尿动力学功能的关系。结果 在逼尿肌细胞中检测到了糖原，随着糖原含量的增加它出现的位置从近浆膜处通过肌层向靠近膀胱上皮处延伸；梗阻时间、膀胱湿重总体上随糖原评分增加呈增加趋势，差异有统计学意义（P＜0.05）；膀胱最大压力、容量方面，2分组、3分组比起0分组、1分组值的增加有统计学意义（P＜0.05），而3分组和2分组的差异没有统计学意义（P﹥0.05）；膀胱顺应性方面,2分组比起1分组值的减小差异有统计学意义（P＜0.05），而3分组和2分组相比值的减小差异没有统计学意义（P﹥0.05）。 结论 近浆膜的逼尿肌层内是否有糖原沉积对于膀胱功能恢复的潜力是个标志；此实验结果可能做为膀胱逼尿肌功能分析的一个重要参数。 关键词 膀胱；梗阻；糖原；尿动力学；缺血 中图分类号：R694 Change of glucogen in detrusor of rats with partial bladder outlet obstruction and their implication LI Haiyang﹡, ZHONG Ganping, WANG Zhiping， WANG Daoying, SHI Tingkai ,MA Junlin (﹡ Department of Urology, Second Hospital of Lanzhou University,Lanzhou 730030,China;) Abstract 0bjective：To explore the change of glucogen in detrusor of rats with partial bladder outlet obstruction(PBOO) and their implication. Material and Methods：A PBOO rat model was created，a control group had a sham operation for comparison. Individual urodynamic data were obtained weekly, then were killed at different levels of bladder dysfunction. Bladder sections were stained with Periodic Acid-Schiff (PAS) to assess overall glycogen granule density and disposition location, scored from 0 to 3. Results were demonstrated with the 5%，25%，75% ，95% percentile. To analysis the results with the methods of Mann -Whitney U test. Results：Glycogen granules were seen in the detrusor,as their number increased their location expanded from only close to the serosa (glycogen score 1) through the detrusor (score 2) up to the urothelium (score 3); The week of obstruction and bladder humid weight were increased accompany with the glycogen score increased (P﹤O.05）;The max bladder pressure and volume of the score 2 and 3 group were higher than that in score 0 and 1 group（P﹤O.05）, there was no statis