HPLC测定氟康唑莫西沙星眼用即型凝胶的含量.PDFVIP

HPLC 测定氟康唑莫西沙星眼用即型凝胶的含量 吴明钗1 1 1 1 1 1 2 ，沈秀微 ，林洁 ，谢丽晓 ，黄鹏 ，易林高 ，林思思 (1.温州医学院附属第三医院，浙江 瑞安 325200 ； 2.温州医学院，浙江 温州 325000) 摘要：目的 建立高效液相色谱法测定氟康唑莫西沙星眼用即型凝胶含量，同时测定制剂中氟康唑、莫西沙星和羟苯乙 酯的含量。方法 采用ODS-C 1 18 色谱柱，以三乙胺缓冲液- 甲醇为流动相，流速1.0 mL·min ，检测波长260 nm 。结果 氟 康唑、莫西沙星、羟苯乙酯的线性范围分别为 25~250 g·mL1(r=0.999 9 ，n=6) ，25~250 g·mL1(r=0.999 9 ，n=6) ， 2.5~25 g·mL1(r=0.999 7 ，n=6) ，平均回收率分别为99.6% ，99.4% ，99.3%。RSD 分别为1.23%，1.20%，1.17%。结论 本 方法操作简便，灵敏度高，重复性好，可用于该制剂的含量测定。 关键词：高效液相色谱法；含量测定；氟康唑；莫西沙星；羟苯乙酯 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2012)06-0534-03 Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC 1 1 1 1 1 1 2 WU Mingchai , SHEN Xiuwei , LIN Jie , XIE Lixiao , HUANG Peng , YI Lingao , LIN Sisi (1.The Third Affiliated Hospital of Wenzhou Medical College, Rui’an 325200, China; 2.Wenzhou Medical College, Wenzhou 325000, China) ABSTRACT: OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C 18 column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25250 μg·mL1(r=0.999 9, n=6), 25250 μg·mL1(r=0.999 9, n=6), 2.525 μg·mL1 (r=0.999 7, n=6), the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation. KEY WORDS: HPLC; content determination; fluconazol