天然橡胶中水溶性蛋白质含量测定及对豚鼠的过敏反应-中国药事.PDF

2010 24 8 759 陈 芳, 杨 敏, 张毅兰(, 201203) : 建立天然橡胶中水溶性蛋白质的含量测定方法, 考察天然橡胶对豚鼠的过敏反应, 并初 探讨降低天然橡胶中蛋白质含量的方法 酸沉淀碱复溶后采用比色法测定蛋白质的含量采用豚 1 鼠评价天然橡胶的过敏反应蛋白质在10~ 200g mL 范围内线性良好 (r= 09996) 豚鼠对天然 橡胶提取液具有明显的过敏反应症状; 对清洗液无过敏症状该方法准确重现性好, 可用于测定天然 橡胶中水溶性蛋白质的含量水溶性蛋白质会引起豚鼠的过敏反应, 但可以通过反复清洗降低或避免 : 天然橡胶; 水溶性蛋白质; 过敏反应 : 9272; 965 : A :(2010) Determination of Water Soluble Protein in Natural Rubber and Its Allergic Reaction to Guinea Pig Chen Fang, Yang Min and Zhang Yilan( SFDA Pharmaceutical Packaging Material esearch and Inspection Center, Shanghai 201203) ABSTRACT: Objecti e To establish the method for determination of water soluble protein in natural rubber, evaluate the allergic reaction to guinea pig and preliminarily research the reduction methods of protein level Methods Use colorimetry to quantitate the protein after acid precipitation and redissolution 1 Evaluate the allergic reaction to guinea pig Results The good linearity range was 10~ 200g mL (r = 09996) The guinea pig had significant allergic reaction to extracting solution of natural rubber while no reaction to washing solution Conclusion The method is accurate, reliable and reproducible It can be used to determine the amount of water soluble protein in natural rubber The water soluble protein would cause allergy, but which can be reduced or avoided by washing the rubber repeatedly KEY WORDS: natural rubber; water soluble protein; allergic reaction ( Sigma , 98% , : , , 113K7001) ; Folin ( SigmaAldrich , [ 13] ( SFDA) : 106K0127) 12 ( A B 2005 ) ( 250~ 350g, ) ,