鹅MC4R基因RFLP及其与胴体和羽绒性状的关联性.pdfVIP

畜牧兽医学报 2008，39(7)：885—890 Acta Veterinaria et Zootechnica Sinica 鹅MC4R基因RFLP及其与胴体和 羽绒性状的关联性 陈宏权 ，黄华云，陈 华，张同燕 (安徽农业大学动物科技学院，合肥230036) 摘 要：本研究利用皖西白鹅为父本与四JlI白鹅(母本)进行杂交，屠宰测定了123只杂种鹅的胴体性状、羽绒性状 和肉质性状．运用因子分析从这些性状中提取了12个公共因子；采用4种内切酶HiM I、Ear I、Pst I和Pvu II检测 了鹅黑素皮质素受体一4(MC4R)基因编码区的RFI P，并分析了RFLP与鹅重要经济性状之间的关联性。结果显 示，试验鹅群中仅 HiM I酶切MC4R基因编码区存在RFI P，酶切得到 HH和Hh 2种基因型，其1对等位基因H 和h的频率分别为0．890 2和0．109 8。分析不同基因型与鹅胴体和羽绒性状之关联性表明，HH型与鹅屠体重、 半(全)净膛重、肝脏重、心脏重、肌胃率、腹脂率、胸肌失水率以及绒比重具有显著的相关性，基因型效应对群体均 数的贡献率在±5 之间；当MC4R基因突变后，Hh型的这种效应未能达到显著水平，但却与产脂力(3．314 )和 脂肪酸品质(1．218 )(PO．05)2个公共因子存在显著的相关性。 关键词：鹅；MC4R基因；RFLP；经济性状 中图分类号：$835．2 文献标识码：A 文章编号：0366—6964(2008)07-0885—06 Restriction Fragment Length Polymorphism in MC4R Gene and Its Association with Carcass and Feather—down Traits in Geese CHEN Hong—quan ，HUANG Hua—yun，CHEN Hua，ZHANG Tong—yan (College of Animal Science and Technology，Anhui Agricultural University，Hefei 230036，China) Abstract：The carcass traits，meat quality traits and feather—down traits of 1 23 geese from F1 pop— ulation of Wanxi White goose(male parent)and Sichuan White goose(female parent)were men— surated，and 1 2 common factors were extracted from the traits using factor analysis．The coding region of melanocortin receptor一4(MC4R)gene was cloned，and the amplified products were di— gested with four restriction endonuc1eases Hinf I，Ear I，Pst I and Pvu II to detect restriction fragment length polymorphism (RFLP)in MC4R gene and its association with the traits．The re— sults showed that the RFLP emerged only using Hinf 1 with tWO genotypes H H and Hh，and frequencies of allele H and h were 0．890 2 and 0．109 8 in F1 population respectively．The effects of ge