桃拉综合征病毒中国株zhzc3 全基因测序及分子 - virologica sinica.pdfVIP

第 21 卷 3 期 中 国 病 毒 学 21 （3 ）：267-272 2006 年 5 月 VIROLOGICA SINICA May 2006 桃拉综合征病毒中国株 ZHZC3 全基因测序及分子结构预测 黄新新，陆承平** （南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京 210095 ） Genome Sequencing and Structure Prediction of ZHZC3 Strain of Taura Syndrome Virus Isolated in China ** HUANG Xin-xin ，LU Cheng-ping （Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095） Abatract: Eight overlapping clones covering the whole viral genome of TSV ZHZC3 isolate were amplified by RT-PCR and the 5′ and 3′ ends were amplified with RACE. The PCR products were cloned into pMD-18T vector and sequenced. The result showed that the genome of ZHZC3 consisted of 10202nt excluding the poly (A) tail, and contained two ORF (ORF 1-2) which encoded polyproteins of S S 2107aa and 1011aa. No insertions or deletions were detected in the coding regions while 3nt of A were deleted at 5′ UTR when compared with HI94. The overall nucleotide sequence identity between ZHZC3 and HI94 was 97.6%. Compared with HI94 and BLZ01, the ORF1 of ZHZC3 shared 97.6% and 97.7% nucleotide sequence identity. The ORF2 shared 98.3 and 97% sequence identity at the nucleotide level. Compared with partial sequences of ORF2 from foreign isolates, both ZHZC3 and the Taiwan isolates shared higher nucleotide identity with HI94. Amino acid sequence analysis of six TSV isolates from differe