invitrogen引物设计参考标准（invitrogen引物设计参考标准）.docVIP

invitrogen引物设计参考标准（invitrogen引物设计参考标准） Invitrogen primer design reference standard Primers designed to provide free design services Primers that do not provide design services lead matter class type Common PCR primers RT-PCR primers (not trans exon) Fluorescent quantitative PCR primers (SYBR, GREEN) (not trans exon) Taqman probes and primers (not trans exon) Common primers with simple restriction sites at both ends Simple hybridization probes 1 、 multi sequence homologous sequence universal PCR and fluorescent quantitative primers 2 and multiple sequence homologous universal Taqman probes and primers 3 methylation primers 4 、 degenerate primers 5. Fusion protein was used to construct primers (linker was added in the middle) 6 nested PCR primers 7. Fluorescence quantitative and RT-PCR primers designed across exons 7. Primer evaluation Hold Thats ok mark accurate In order to ensure the timely design, each customer only for a template free design, primer design good only to ensure that the appropriate parameters, does not guarantee the specificity and the experimental results, we do not help the screening and BLAST, did not provide consulting and technical support. If you are in urgent need of design and the project staff will not be able to respond soon, please use the following online design yourself: / Because of the complicated design, project personnel will take a lot of time, interfere with the normal projects, but also to ensure completion in a relatively short period of time, delay your valuable time, therefore, the design above is complex and takes a lot of time, from January 2008 14 onwards, we do not provide design services. Thank you for your understanding and support! prepare notes 1. for primer design, we only accept sequences that have been checked and identified. We do not provide help to find sequences, and to help determine the sequence of services. 2. for free design primers, we will give the design within three working days, we do not accept telepho