离子液体复合磁性纳米粒子的制备及其在DNA提取中的应用乔晋东.pdfVIP

网络出版时间：2016-04-22 16:14:20 网络出版地址：/kcms/detail/11.1347.D1614.016.html 刑事技术 2016 年 第41 卷 第2 期 ·技术与应用· DOI: 10.16467/j.1008-3650.2016.02.016 离子液体复合磁性纳米粒子的制备及其在DNA提取中的应用 乔晋东 , 张小恺 , 庞 飞 , 冯鹏飞 （秦皇岛市公安局刑警支队，河北 秦皇岛 066000） 摘　要：目的研究制备离子液体复合磁性纳米粒子，提高 DNA 提取纯化效率。方法将具有高提取效率的离子 液体作为功能单体，乙二醇二甲基丙烯酸酯为交联剂，磁流体为分散相，聚乙烯吡咯烷酮为致孔剂，偶氮二异 丁腈为引发剂，采用一步悬浮聚合法制备出具有较高提取效率的超顺磁性纳米粒子，并与 QIAGEN 磁珠试剂 盒进行比较。结果制备的离子液体复合磁性纳米粒子化学性质稳定、分散性能良好、粒径均匀且具有超顺磁性， 优于 QIAGEN 磁珠试剂盒。结论 离子液体复合磁性纳米粒子可用于微量生物检材 DNA 提取，操作过程简单 方便、所用试剂环境友好、成本低廉。 关键词：DNA 提取；离子液体复合磁性纳米粒子；一步悬浮聚合法 中图分类号： DF795.2 文献标识码：A 文章编号：1008-3650(2016)02-0155-03 Preparing Ionic Liquid Composite Magnetic Nano-beads to Extract DNA QIAO Jindong, ZHANG Xiaokai, PANG Fei, FENG Pengfei (Criminal Police Detachment of Qinhuangdao Municipal Public Security Bureau, Hebei, Qinhuangdao 066000, China) ABSTRACT: Objective Aiming at the preparation of the ionic liquid composite magnetic nano-beads and their application into the trace DNA extraction to both improve the extraction efficiency and reduce the cost and requirements of the traditional magnetic-bead method. Methods The magnetic nano-beads of high extraction efficiency and superparamagnetism were synthesized with one step suspension polymerization by an ionic liquid as the monomer, ethylene glycol dimethacrylate as the cross-linker, magnetic fluid as the dispersant solvent, polyvinylpyrrolidone as the porogen, and the azodiisobutyronitrile as the initiator. The prepared ionic liquid composite magnetic nano-beads were compared with the QIAGEN kit for their efficiency to extract DNA sampl