多枝雾水葛不同提取部位的抗炎镇痛作用研究.pdfVIP

• 356 • 现代药物与临床 Drugs Clinic 第 27 卷 第 4 期 2012 年 7 月 多枝雾水葛不同提取部位的抗炎镇痛作用研究 刘旭阳，杜清涛，李开莹，邓 琪，郭丽冰* 广东药学院 中药学院，广东 广州 510006 摘 要：目的 研究多枝雾水葛不同提取物的抗炎镇痛作用。方法 采用二甲苯致小鼠耳肿胀、醋酸致小鼠扭体反应和小鼠 热板法观察多枝雾水葛灌胃、外搽使用以及不同提取部位的抗炎、镇痛作用。结果 多枝雾水葛外搽使用的抗炎镇痛效果不 明显，灌胃给药具有较强的抗炎镇痛作用，能显著减少醋酸所致的小鼠扭体次数和抑制二甲苯所致的小鼠耳廓肿胀，且其水 提物比醇提物的作用更强。对多枝雾水葛水提取物的活性部位研究表明，多枝雾水葛的氯仿部位和水部位都具有较好的镇痛 效果，抗炎的有效部位则是氯仿部位和正丁醇部位。结论 多枝雾水葛水提取物灌胃给药具有抗炎镇痛作用，其有效部位是 氯仿部位、正丁醇部位和水提取部位。 关键词：多枝雾水葛；抗炎；镇痛；耳肿胀；扭体反应；热板法 中图分类号：R285.5 文献标志码：A 文章编号：1674 - 5515(2012)04 - 0356 - 04 Anti-inflammatory and analgesic effects of different extract fractions from Pouzolzia zeylanica var. microphylla LIU Xu-yang, DU Qing-tao, LI Kai-ying, Deng Qi, GUO Li-bing School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China Abstract: Objective To study the anti-inflammatory and analgesic effects of different extracts from Pouzolzia zeylanica var. microphylla. Methods The xylene-induced ear edema of mice, acetic acid-induced writhing of mice, and hot-plate procedure in mice were used. The anti-inflammatory and analgesic effects of P . zeylanica var. microphylla by ig administration and external use were observed, and the effects of different extract fractions from P . zeylanica var. microphylla were studied. Results There were no obvious anti-inflammatory and analgesic effects of P . zeylanica var. microphylla by external use, while ig administration had stronger analgesia by significantly reducing the number of body torsion and inhibiting xylene-induced ear edema of mice. The water extracts had much better effect than the ethanol extracts. According to the study on the active fractions of water extract from P . zeylanica var. microphylla, it showed that both chloroform and water fractions had better analgesic effects, while the