巢蛋白过表达对小鼠肝脏增殖的影响 - 中国实验动物学报.docVIP

Nestin增强部分肝切除小鼠模型肝再生增殖能力的研究 纪小珍1，李振林2，贾俊双1，安靓1 （1.南方医科大学肿瘤研究所,广州 510515；2.南方医科大学组织胚胎教研室，广州 510515） 【摘要】 目的 探讨巢蛋白（nestin）基因过表达对小鼠部分肝切除后肝再生增殖能力的影响及其可能的分子机制。方法 应用荧光定量PCR、Western blot和免疫组化的方法检测转基因小鼠肝脏中外源基因nestin的表达情况。使用Brdu标记法比较转基因小鼠和野生型小鼠肝脏在不同状态下（静息状态、肝叶切除后24h、肝叶切除后72h）肝细胞增殖能力的差异。结果 荧光定量PCR、Western blot和免疫组化证实转基因小鼠肝脏中存在nestin的高表达状态。在静息状态下，野生鼠和nestin过表达的转基因鼠肝脏BrdU标记率几乎为零,两者无明显差异；但在肝大部切除的情况下，nestin过表达的转基因鼠有更多的细胞进入增殖状态，增殖能力较野生鼠明显增强，差别具有统计学意义（24h ：t=2.996,P=0.024; 72h：t=2.915，P=0.027）。结论 Nestin过表达对静息状态下的肝脏增殖无影响，但可增加损伤修复情况下肝脏的再生增殖能力。表明nestin过表达对静止期细胞作用不明显，但在一些病理或刺激状态下nestin可以通过促进DNA的合成而加速细胞的分裂增殖。 【关键词】Nestin；肝大部切除；肝脏增殖；小鼠 【中图分类号】R-332 【文献标志码】 A The research of nestin increase the regeneration and proliferation capacity in the case of partial hepatectomy in mice JI Xiao-zhen1,LI Zhen-lin2,JIA Jun-shuang1,AN Jing1 (1.Cancer Research Institute,Southern Medical University,Guangzhou 510515,China;2.Department of Embryology Histology,Southern Medical University,Guangzhou 510515,China) 【Abstract】Objective To study the influence and possible mechanism of nestin overexpression on the partial hepatectomy regeneration and proliferation capacity in mice .Methods In order to confirm the overexpression status,the expression levels of nestin in liver was detected by fluorescence quantitative PCR,Western blot and immunohistochemistry.Then,The difference of proliferation index in wild type mice and transgenic mice liver was detected by Brdu label,the mice were divided into 3 groups(in the resting state,24hours later of liver subtotal nephrectomy,72hours later of liver subtotal nephrectomy).Results Fluorescence quantitative PCR,Western blot and immunohistochemistry confirmed overexpression of nestin in the liver of the transgenic mice.In the resting state,Brdu labelling index is almost zero both in the transgenic mice and the wild type mice,showing no difference between them;However,in the case of partial hepatectomy,more cells in the transgenic mice enter into proliferation status,suggesti