苹果snrk2基因家族的鉴定和生物信息学分析 - 中国农学通报.pdfVIP

中国农学通报 2013,29(13): 120-127 Chinese Agricultural Science Bulletin 苹果SnRK2 基因家族的鉴定和生物信息学分析 陈娜娜，刘金义，蔡 斌，王 刚，王 敏，程宗明 （南京农业大学园艺学院，南京210095 ） 摘 要：SnRK2 是植物特有的一类Ser/Thr 类蛋白激酶，在植物逆境生理过程中扮演了重要的角色。利用 隐马可夫模型有哪些信誉好的足球投注网站和系统建树的方法，在苹果基因组中鉴定出了16 个SnRK2 基因家族成员，然后从系 统发生、理化性质、二级结构、motif 、C 末端序列和EST 等方面对其进行了预测和分析。结果表明，苹果 SnRK2 家族为亲水性蛋白，二级结构主要以α-螺旋和不规则卷曲为主；在系谱发生上与拟南芥、水稻、玉 米所有的SnRK2 蛋白一起分成了3 个亚族；motif 和C 末端序列分析显示，苹果SnRK2 蛋白激酶家族具 有较高的保守性；EST 表达分析显示，16 个MdSnRK2 在不同组织中呈不均匀分布，具有组织特异性。 本研究为苹果SnRK2 基因家族生物学功能的研究奠定了生物信息基础。 关键词：苹果；SnRK2 ；蛋白激酶；基因组 中图分类号：S661.1 文献标志码：A 论文编号：2012-3447 Identification and Bioinformatics Analysis of the SnRK2 Gene Family in Apple (Malus×domestica Borkh.) Chen Nana, Liu Jinyi, Cai Bin, Wang Gang, Wang Min, Cheng Zongming (CollegeofHorticulture,NanjingAgriculturalUniversity, Nanjing 210095) Abstract: SnRK2 is a class of Ser/Thr protein kinase, which is unique to plants, playing important roles in regulating stress physiological process. We employed the method of HMMER and phylogenetic tree, identified 16 SnRK2 members in apple genome, then we predicted and analyzed the evolutionary relationship, physical and chemical characteristics, secondary structures, motif, C-terminus sequences and EST. The result showed that the genes identified in apple were hydrophilic protein, secondary structure were mainly α-helix and irregular curl; They were classified to three subfamilies; motif and C-terminus sequences analysis showed that apple SnRK2 protein kinases were highly conserved; EST analysis showed that 16 MdSnRK2 were uneven distribution in different tissues, having tissue-specific. This research provided a bioi