荧光增白剂和增效蛋白对粘虫核型多角体病毒的增效作用.pdf

华北农学报2008, 23( 4) : 23-27 1,2 2 2 2 1,2 陈 涛 , 王俊平, 李淑文 , 周洪旭, 李国勋 ( 1. , , 071001; 2. , 266109) :, SDS FB28 ( En-Ha) (Ms PV),FB28 , 1% FB28 4 h ,, , En-Ha , ,, ; 9 g/mL En-Ha 7 h,,,FB28 En-Ha , , FB28 En-Ha Ms PV , LC 50 :; ;; ; + :S432.4 1 :A : 1000- 7091(2008)04- 0023- 05 Enhancing Effects of Fluorescent Brightener 28 and Enhancin on Mythimna sep arata Nucleopolyhedrovirus 1, 2 2 2 2 1, 2 CHE Tao ,WA G Jun-ping , LI Shu-wen ,ZHOU Hong-xu , LI Guo-xun ( 1.Center for Biocontrol Techniqueof Agricultural Pest in Hebei Province,Hebei AgriculturalUniversity,Baoding 071001,China;2.Institutefor Biocontrol of Insect Pests,Qingdao AgriculturalUniversity,Qingdao 266109, China) Abstract:The disruptive effects of Fluorescent Brightener 28(FB28) and H eliothis armigera granulovirus enhancing (En-Ha) on theperitrophicmembrane(PM) of larvae and enhancing effectsonMs PV in Mythima sep arata were studied through SDS,structural observation and bioassayThe results indicated that proteins could be dissociated from the PM in vitro by FB28After the larvae were reared with diets containing 1% FB28for 4 h, bluish fluorescence appeared on the disrupted PMSDSof PM treatedwith FB28 in vivo showed that some proteins with high molecular weight disappearedAfter the PM was treated with En-Ha in vitro , some proteins with high molecular weight disappeared and some with lower molecular weight appeared,which indicated En-Ha could degradethehigh-molecular-weight proteins from the PMThe PM becamefragile after the larvaewere rearedwi