二十二碳六烯酸-磷脂脂质体对S180荷瘤小鼠肿瘤抑制和免疫调节作用.pdfVIP

中囤善科太擎学报 Journal of China Pharmaceutical University 2008，39(2)：159—163 159 二十二碳六烯酸一磷脂脂质体 对S180荷瘤小鼠 肿瘤抑制和免疫调节作用 马 琴，王静凤，王 琦，高 森，赵 芹，刘 鹤，薛长湖 (中国海洋大学食品科学与工程学院，青岛266003) 摘 要 目的：探讨二十二碳六烯酸(DHA)一磷脂的抗肿瘤和免疫调节作用。方法：从鸢乌贼卵(Symplectoteuthis oual— aniensis)中提取制备DHA一磷脂，并制备单室脂质体；采用不同剂量的DHA一磷脂脂质体灌胃移植性肿瘤模型小鼠，分别测定 其瘤重、脾脏指数、胸腺指数、血清溶血素含量、足跖增厚值和巨噬细胞吞噬能力。结果：DHA一磷脂脂质体能显著抑制小鼠 S 肉瘤的生长，其平均抑瘤率为36．87％，提高荷瘤小鼠脾脏脂数(P0．05)，显著提高血清溶血素含量(P0．05，P 0．01)，提高迟发型变态反应水平(P0．05，P0．01)，促进巨噬细胞吞噬能力(P0．01)。结论：DHA一磷脂脂质体能全面 调节机体的特异性和非特异性免疫功能，抑制肿瘤细胞的生长。 关键词 二十二碳六烯酸；磷脂；脂质体；抗肿瘤；免疫调节 中图分类号 R965 文献标识码 A 文章编号 1000—5048(2008)02—0159—05 Ants-tumor activities and immune regulation of docosahexaenoic acidI phosphatidylcholine liposomes in mice bearing S18o MA Qin，WANG Jing—feng，WANG Qi，GAO Sen，ZHAO Qin，L1U He，XUE Chang—hu College of Food Science and Technology，Ocean University of China，Qingdao 266003，China Abstract Aim：T0 investigate the anti．tumor activities and immune regulation of the docosahexaenoic acid (DHA)一ph0sphatidylch0line(PC)liposomes in S180-bearing mice．Methods：DHA—PC(containing 30％DHA) separated from cuttlefish spawns was used to prepare unilayer liposomes．Different doses of DHA—PC liposomes were given to the transplanted tumor．bearing mice，and the tumor weight，spleen index，thymus index，hemolysin content，delayed type hypersensitivity and the phagocytosis ability of peritoneal macrophage were measured．Re。 suits：It was shown that administration of DHA．PC liposomes inhibited the growth of tumor in S180一bearing mice and that the average tumor．inhibitory rate was 36．87％．Furthermore．there were increases of the spleen index fP0．05)，the hemolysin content fP0．05，P0．01)，delayed allergic level(P0．05，P0．01)，and phagocytosis capability of peritoneal macrophage(P0．01)．Conclusion：DHA—PC liposomes could regulate both the spe．cific and non．specific immune functions，and inhi