·专论·中药小分子单克隆抗体技术平台的构建.pdf

中草药 Chinese Traditional and Herbal Drugs 第45 卷 第7 期 2014 年4 月 ·895 · ·专 论 · 中药小分子单克隆抗体技术平台的构建 * * 屈会化，赵 琰 ，王庆国 北京中医药大学 “经典方剂的应用基础研究”创新团队，北京 100029 摘 要：抗体是现代生命科学研究中极其重要的工具，小分子抗体技术在中医药研究领域具有广阔的应用前景。结合创新团 队开展的研究工作，介绍了中药小分子单克隆抗体技术平台建立的背景和抗体制备的技术难点，针对基于中药小分子单克隆 抗体的技术产品，如 ELISA 检测试剂盒、免疫亲和色谱柱、胶体金试纸、荧光标记抗体和抗体芯片等，探索实践了其在中 医药研究领域中的多种应用，并对中药小分子单克隆抗体技术的科学意义和应用价值进行了展望。 关键词：中药小分子；单克隆抗体制备；酶联免疫吸附法；免疫亲和色谱柱；胶体金试纸；荧光标记抗体；抗体芯片 中图分类号：R285 文献标志码：A 文章编号：0253 - 2670(2014)07 - 0895 - 05 DOI: 10.7501/j.issn.0253-2670.2014.07.001 Establishment of small molecular monoclonal antibody technology platform in Chinese materia medica QU Hui-hua, ZHAO Yan, WANG Qing-guo “Classical Prescription Application Foundation Research” Innovation Team, Beijing University of Chinese Medicine, Beijing 100029, China Abstract: Antibody is a tool of vital importance in modern bioscience research, small molecular antibody technology has a broad application prospect in the field of receptor binding analysis, enzyme assays, and quantitative and/or qualitative analytical techniques of Chinese materia medica (CMM) research. In this paper, combining with the research work carried out by our innovation team, we introduced the establishment background of the small molecular monoclonal antibody technology platform in CMM and technical difficulties in antibody preparation. In view of the technology products based on small molecular monoclonal antibody of CMM, such as ELISA test kit, immune affinity chromatography column, colloidal gold test paper, fluorescently labeled antibodies, and antibody microarrays, we explored and practised the various applications based on the small molecular monoclonal antibody technology looking forward to its scientific significances and application values in the field of CMM re