原子吸收分光光度法测定富硒决明子中的硒含量-中国中医药信息杂志.PDF

2008年 3月第 15卷第 3期 中国中医药信息杂志 ·39· ·中药研究与开发· 原子吸收分光光度法测定富硒决明子中的硒含量 1 2 1 张 玲 ,李 青 ,夏作理 (1.泰山医学院生命科学研究所,山东 泰安 271000；2.山东农业大学校医院,山东 泰安 271018) 摘要：目的 探讨富硒决明子中硒含量的测定方法。方法 应用石墨炉原子吸收分光光度法测定富硒决明子芽 中硒含量。结果与结论 本实验筛选出了适合于测定决明子中硒含量的方法和条件；富硒决明子消化后,用加入基体 改进剂的含0.1%土温80的1%稀硝酸溶解定容,测定结果准确可靠。 关键词：原子吸收分光光度法；硒；决明子 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2008)03-0039-02 Determination of Elements Seleninum in Se Semen Cassiae by Graphite Atomic Absorption 1 2 1 Spectrophotometer ZHANG Ling , LI Qing , XIA Zuo-li (1.Institute of Life Sciences, Taishan Medical College, Taian 271000, China；2.School Health Care of Shandong Agriculture University, Taian 271018, China) Abstract ：Objective To explore the determination of elements selenium content in Se Semen Cassiae. Method Selenium content in Se Semen Cassiae was measured by graphite atomic absorption spectrophotometer. Result and Conclusion An appropriate extraction method and digesting condition were screened out. The determined results were accurate when the digested sample was dissolved and metered volume with 1% HNO3 which containing 0.1% Tween 80. Key words：graphite atomic absorption spectrophotometer；selenium；Semen Cassiae — 表7 注射用银黄冻干粉针对恢复期雄性大鼠血液生化指标的影响(x±s,每组 n =5) ALT AST ALP Glu T-BIL BUN Cr ALB TP TCHO 组别 (U/L) (U/L) (U/L) (mmol/L) (µmol/L) (mg/dL